Studies on formulation development and evaluation of tolnaftate-loaded glycerosomes
Yasmin Begum Mohammed, Ali Alqahtani, S. Mohana Lakshmi, K Gnanaprakash, CT Kumarappan
Abstract
Purpose The aim was to formulate and evaluate glycerosomes as novel carrier for dermal (trans) drug delivery systems (tDDDS).Methods Tolnaftate Loaded Glycerosomes were prepared by thin film hydration technique. The thin-film was hydrated using aqueous solution of glycerol (30 %v/v) against liposomes as control and were characterized.Results Glycerosome formulations were sphere-shaped with a mean diameter of 165.5 nm and narrow size distribution (P.I: 0.744). Zeta potential was –22.8mv indicating good stability. Tolnaftate loading capacity was found to be between 17.5±0.56 % and 91.32±0.57%. In-vitro permeation experiments showed an improved skin deposition and permeation of tolnaftate (5mg) when 30% glycerol, DSPC (100mg), cholesterol (25mg) content were used. Ex-vivo skin penetration studies showed an improved drug release than conventional liposomes and drug suspension. Skin irritancy studies on guinea pig showed no signs of toxicity.Conclusion Glycerosomes must be an ideal novel transdermal drug delivery system for the effective delivery of tolnaftate.