Single nucleus RNA-sequencing: how it's done, applications and limitations
Juliane Fischer, Thomas Ayers
Abstract
Single nuclei RNA-sequencing (sNuc-Seq) is a methodology which uses isolated nuclei instead of whole cells to profile gene expression. By using droplet microfluidic technologies, users are able to profile thousands of single transcriptomes at high throughput from their chosen tissue. This article aims to introduce sNuc-Seq as a method and its utility in multiple tissue types. Furthermore, we discuss the risks associated with the use of nuclei, which must be considered before committing to a methodology.
Topics & Concepts
NucleusRNAComputational biologyRNA-SeqTranscriptomeComputer scienceThroughputBiologyGeneGene expressionCell biologyGeneticsTelecommunicationsWirelessSingle-cell and spatial transcriptomicsCancer Genomics and DiagnosticsExtracellular vesicles in disease