Integrating a Far‐Red Fluorescent Probe with a Microfluidic Platform for Super‐Resolution Imaging of Live Erythrocyte Membrane Dynamics**
Zhiwei Ye, Wei Yang, Ying Zheng, Shujing Wang, Xiaodong Zhang, Haibo Yu, Shuangshuang Li, Chunxiong Luo, Xiaojun Peng, Yi Xiao
Abstract
Living erythrocyte (red blood cell, RBC) membranes present rich ultrastructural and dynamic details, which require synchronous super-resolution imaging and single-molecule tracking to be revealed. Yet, it poses a serious challenge to achieve these dual functions in a single probe, due to the rigid and conflicting photophysical demands of the different techniques. Herein, we rationally developed a far-red boron dipyrromethene membrane probe with blinking capability and persistent single-molecule emission, and constructed a microfluidic platform for noninvasive trapping and long-term imaging of RBCs. By combining them, multi-dimensional super-resolution reconstructions and single-molecule tracking were achieved at the molecular scale on living human RBC membranes in a high-throughput manner. Our integrated system defines a quantitative method for analyzing RBC membranes under physiological and pathological conditions, improving precision and revealing new perspectives for future disease diagnostics.