A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers
Mohamed A. Nasr, Logan R. Timmins, Vincent J. J. Martin, David H. Kwan
Abstract
. In addition to salicylate, we demonstrate the responsiveness of CmeR-regulated promoters to multiple aromatic and indole inducers. This relaxed ligand specificity of CmeR makes it a useful tool for detecting molecules in many metabolic engineering applications, as well as a good target for directed evolution to engineer proteins that are able to detect new and diverse chemistries.
Topics & Concepts
TetRSynthetic biologyBiosensorComputational biologyMetabolic engineeringBiologyIndole testTranscription factorProtein engineeringDirected evolutionRepressorBiochemistryGeneEnzymeMutantMicrobial Metabolic Engineering and BioproductionRNA and protein synthesis mechanismsBacterial Genetics and Biotechnology