Plasma-Derived HIV-1 Virions Contain Considerable Levels of Defective Genomes
Katie Fisher, Xiao Qian Wang, Ashley Lee, Vincent Morcilla, Anneke de Vries, Eunok Lee, John‐Sebastian Eden, Steven G. Deeks, Anthony D. Kelleher, Sarah Palmer
Abstract
-3' PRLS assay, we found that analytical treatment interruption (ATI) plasma-derived sequences were identical and genetically-intact. Several sequences from the ATI plasma samples were identical to viral sequences from pretherapy plasma and PBMC samples, indicating that HIV-1 reservoirs established prior to therapy contribute to viral rebound during an ATI. Therefore, near-full-length sequencing of HIV-1 particles is required to gain an accurate picture of the genetic landscape of plasma HIV-1 virions in studies of HIV-1 replication and persistence.
Topics & Concepts
GenomeBiologyRNAVirologyDNAPeripheral blood mononuclear cellDeep sequencingDNA sequencingVirusIntegraseLentivirusGeneticsHuman immunodeficiency virus (HIV)Molecular biologyComputational biologyGeneViral diseaseIn vitroHIV Research and TreatmentImmune Cell Function and InteractionRNA Interference and Gene Delivery