Reliance on Cox10 and oxidative metabolism for antigen-specific NK cell expansion
Annelise Y. Mah-Som, Molly P. Keppel, Joshua M. Tobin, Ana Kolicheski, Nermina Saucier, Veronika Sexl, Anthony R. French, Julia A. Wagner, Todd A. Fehniger, Megan A. Cooper
Abstract
NK cells and impaired NK cell memory formation. Proliferation in vitro and homeostatic expansion were intact, indicating a specific metabolic requirement for antigen-driven proliferation. Cox10-deficient NK cells upregulated glycolysis, associated with increased AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) activation, although this was insufficient to protect the host. These data demonstrate that oxidative metabolism is required for NK cell antiviral responses in vivo.
Topics & Concepts
Cell biologyAMPKBiologyOxidative phosphorylationDownregulation and upregulationPI3K/AKT/mTOR pathwaySignal transductionChemistryProtein kinase AKinaseBiochemistryGeneImmune Cell Function and InteractionCytomegalovirus and herpesvirus researchT-cell and B-cell Immunology