Litcius/Paper detail

Evaluation of the ResistancePlus MG FleXible Assay for Detection of Wild-Type and 23S rRNA-Mutated Mycoplasma genitalium Strains

Suhella M. Tulsiani, Peter Njuguna, Samantha Ebeyan, Simon Erskine, Mette Holm, Susanne Cramer Johansson, Lit Yeen Tan, Jørgen Skov Jensen

2020Journal of Clinical Microbiology20 citationsDOIOpen Access PDF

Abstract

Antibiotic resistance in Mycoplasma genitalium is rising globally, and resistance-guided diagnostics can facilitate targeted and timely treatment. The ResistancePlus MG FleXible (RPMG Flex) assay for the detection of M. genitalium and macrolide resistance-mediating mutations (MRMM) was evaluated for analytical sensitivity, specificity, reproducibility, and inhibition in the presence of interfering substances by simulating M. genitalium -negative pooled urine and swab matrices with M. genitalium cultures.

Topics & Concepts

Mycoplasma genitalium23S ribosomal RNAMicrobiologyVirologyBiologyMycoplasmaAntibioticsChlamydia trachomatisGeneGeneticsRNARibosomeMicrobial infections and disease researchBlood groups and transfusionReproductive tract infections research