Conjoint analysis of lncRNA and mRNA expression in rotator cuff tendinopathy
Zilu Ge, Hong Tang, Jingtong Lyu, Binghua Zhou, Ming‐Yu Yang, Kanglai Tang, Wan Chen
Abstract
BACKGROUND: Rotator cuff tendinopathy (RCT) is a common musculoskeletal disorder in the shoulder, whose underlying mechanism is unknown. Long non-coding RNAs (lncRNAs) are involved in the development of various diseases, but little is known about their potential roles in RCT. METHODS: In this study, we profiled lncRNAs and mRNAs involved in RCT in comparison with the normal tendon (NT) by RNA sequencing (RNA-Seq), to identify potential therapeutic targets. Gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG) pathway, competing endogenous RNA (ceRNA), and co-expression network construction were used to identify the potential functions of these RNAs. Three lncRNAs and three mRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS: were validated by PCR. We constructed a co-expressed network of these validated RNAs. CONCLUSIONS: We preliminarily analyzed the profile of lncRNAs and mRNAs in RCT. The bioinformatic analysis revealed several potential therapeutic targets for RCT.