Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
Mitchell A. Moseng, Meinan Lyu, Tanadet Pipatpolkai, Przemysław Glaza, Corey C. Emerson, Phoebe L. Stewart, Phillip J. Stansfeld, Edward Yu
Abstract
The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the trimer recognizes and extrudes Cu(I) independently.
Topics & Concepts
TrimerEffluxChemistryBiophysicsMembraneMetal ions in aqueous solutionBacteriaMetalNanotechnologyStereochemistryBiochemistryBiologyMaterials scienceOrganic chemistryGeneticsDimerBacterial biofilms and quorum sensingTrace Elements in HealthAntibiotic Resistance in Bacteria