Study on release of cardamom extract as an antibacterial agent from electrospun scaffold based on sodium alginate
Shima Najafi, Adeleh Gholipour‐Kanani, Niloofar Eslahi, S. Hajir Bahrami
Abstract
In the present study, sodium alginate (SA):polyvinyl alcohol(PVA) blends with different concentrations of cardamom extract (CE) were electrospun. The SEM images show that the optimum content of CE was 10 wt% resulting in fibers with average diameter of 233 ± 33 nm. FTIR spectra was employed to investigate probable interactions between polymers and CE. The CE release follows first order kinetics with 86% initial burst release at the first 24 h necessary to desired antibacterial activity. To investigate the biological properties, MTT assay, cell adhesion and antibacterial test were carried out. The SA:PVA:CE nanofibers showed no toxicity against fibroblast cells. The cell culture results exhibited the proper biocompatibility and proliferation on both CE-loaded and unloaded webs. The antibacterial test showed that CE-loaded nanofibrous web is capable of improving the antibacterial activity about 99% and 97% against gram-positive and gram-negative bacteria, respectively. Therefore, the CE-loaded web can be used as an antimicrobial wound dressing for healing improvement.