UHPLC–HRMS Method for the Simultaneous Screening of 235 Drugs in Capillary Blood for Doping Control Purpose: Comparative Evaluation of Volumetric and Non-volumetric Dried Blood Spotting Devices
Monica Mazzarino, Ludovica Di Costanzo, Fabio Comunità, Carlotta Stacchini, Xavier de la Torre, Francesco Botrè
Abstract
. Stability studies simulating the storage of samples before the analysis and in view of a possible re-analysis showed that most of the analytes were stable for at least 24 h at 50 °C and for at least 3 weeks at 25 and at 4 °C. The real applicability of the method was assessed by analyzing the samples collected after the administration of two model drugs, acetazolamide and deflazacort. The performance of the method was confirmed to be fit for purpose, and data obtained in blood can also be used to complement those available in urine, allowing to refine the knowledge concerning the pharmacokinetic profiles.
Topics & Concepts
SpottingDried bloodCapillary actionBiomedical engineeringChromatographyMedicineMaterials scienceComputer scienceChemistryArtificial intelligenceComposite materialBiosimilars and Bioanalytical MethodsHormonal and reproductive studiesPharmacogenetics and Drug Metabolism