Application of 3D Scaffolds in Tissue Engineering
Seyedeh Mahdieh Khoshnazar, Asadollah Asadi, Saman Roshancheshm, Aida Karimian, Arash Abdolmaleki
Abstract
Abstract— Three-dimensional (3D) scaffolds are often used in tissue engineering applications to produce an environment that is conducive to the integration of cells or growth factors to repair or replace damaged tissues or organs. These scaffolds are utilized to mimic the microenvironment seen in vivo, where cells interact and respond to mechanical cues from their three-dimensional surroundings. Consequently, cellular response and fate depend greatly on the material properties of scaffolds. These three-dimensional scaffolds' porous, networked pore structures enable the movement of nutrients, oxygen, and waste. This article looks at the many manufacturing procedures (such as conventional and rapid prototyping techniques) used to create 3D scaffolds with variable pore sizes and porosities. The various methods for determining pore size and porosity will also be covered. It has also been investigated if scaffolds with graded porosity may more accurately mimic the in vivo situation in which cells are exposed to layers of various tissues with changing characteristics. Following a look at the extracellular matrix, nature’s own scaffold, the ability of scaffold pore size and porosity to affect biological responses and mechanical qualities will also be investigated. We will talk about the problems with the current ways of building scaffolds for tissue engineering applications and offer some new and exciting alternatives.