Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for the Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts
Nerea Moreno, Irene González-Martínez, Rubén Artero, Estefanía Cerro-Herreros
Abstract
Western blot assays are not adequate for high-throughput screening of protein expression because it is an expensive and time-consuming technique. Here we demonstrate that quantitative dot blots in plate format are a better option to determine the absolute contents of a given protein in less than 48 h. The method was optimized for the detection of the Muscleblind-like 1 protein in patient-derived myoblasts treated with a collection of more than 100 experimental oligonucleotides.
Topics & Concepts
Myotonic dystrophyBlotOligonucleotideWestern blotDot blotMolecular biologyProtein expressionQuantitative analysis (chemistry)BiologyChemistryBiochemistryDNAGeneticsChromatographyGeneGenetic Neurodegenerative DiseasesMuscle Physiology and DisordersCardiomyopathy and Myosin Studies