MALDI-2 for the Enhanced Analysis of <i>N</i>-Linked Glycans by Mass Spectrometry Imaging
Bram Heijs, Alexander Potthoff, Jens Soltwisch, Klaus Dreisewerd
Abstract
cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies. In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized. The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.
Topics & Concepts
ChemistryMass spectrometryGlycanFragmentation (computing)IonTandem mass spectrometryAnalytical Chemistry (journal)Mass spectrometry imagingMatrix-assisted laser desorption/ionizationIon trapCollision-induced dissociationChromatographyDesorptionGlycoproteinBiochemistryComputer scienceOperating systemOrganic chemistryAdsorptionMass Spectrometry Techniques and ApplicationsGlycosylation and Glycoproteins ResearchAdvanced Proteomics Techniques and Applications