<p>Long Non-Coding RNA <em>SNHG14</em> Regulates <em>SPIN1</em> Expression to Accelerate Tumor Progression in Non-Small Cell Lung Cancer by Sponging <em>miR-382-5p</em></p>
Xiaoliang Chen, Pingan Song, Yuan Yao, Yang Yang
Abstract
Background: Non-small cell lung cancer (NSCLC) is the most common type of lung carcinoma. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 ( SNHG14 ) was identified to participate in tumor progression. However, the mechanism and functions of SNHG14 were rarely reported in NSCLC progression. Methods: The relative gene expression was tested by qRT-PCR. Cell viability, apoptosis, migration and invasion were measured by MTT assay, flow cytometry, and transwell migration and invasion assays, respectively. The interactions between miR-382-5p and SNHG14 or SPIN1 were predicted by starBase and confirmed by the dual-luciferase reporter assay and RNA pull-down assay. The protein level of SPIN1 was evaluated by Western blot assay. Results: The levels of SNHG14 and SPIN1 were significantly increased, while the level of miR-382-5p was apparently reduced in NSCLC tissues and cells. SNHG14 was verified to sponge miR-382-5p and SPIN1 was identified as a direct target of miR-382-5p . SNHG14 depletion repressed cell viability, migration and invasion, but induced the apoptotic rate by targeting miR-382-5p . miR-382-5p overexpression blocked cell viability, metastasis and promoted cell apoptosis by regulating SPIN1 . SNHG14 silencing down-regulated SPIN1 expression by sponging miR-382-5p . Conclusion: SNHG14 facilitated NSCLC progression by regulating SPIN1 expression via targeting miR-382-5p . Keywords: lncRNA SNHG14 , miR-382-5p , SPIN1 , tumor progression, NSCLC