Following Electroenzymatic Hydrogen Production by Rotating Ring–Disk Electrochemistry and Mass Spectrometry**
Jaloliddin Khushvakov, Robin Nussbaum, Cécile Cadoux, Jifu Duan, Sven T. Stripp, Ross D. Milton
Abstract
Abstract Gas‐processing metalloenzymes are of interest to future bio‐ and bioinspired technologies. Of particular importance are hydrogenases and nitrogenases, which both produce molecular hydrogen (H 2 ) from proton (H + ) reduction. Herein, we report on the use of rotating ring–disk electrochemistry (RRDE) and mass spectrometry (MS) to follow the production of H 2 and isotopes produced from deuteron (D + ) reduction (HD and D 2 ) using the [FeFe]‐hydrogenase from Clostridium pasteurianum, a model hydrogen‐evolving metalloenzyme. This facilitates enzymology studies independent of non‐innocent chemical reductants. We anticipate that these approaches will be of value in resolving the catalytic mechanisms of H 2 ‐producing metalloenzymes and the design of bioinspired catalysts for H 2 production and N 2 fixation.