Epitope-directed antibody selection by site-specific photocrosslinking
Longxin Chen, Chaoyang Zhu, Hui Guo, Runting Li, Limeng Zhang, Zhenzhen Xing, Yue Song, Zihan Zhang, Fuping Wang, Xiaofeng Liu, Yuhan Zhang, Z. Runlin, Feng Wang
Abstract
-benzoyl-l-phenylalanine (pBpa), on the target antigen epitope. By one or two rounds of panning against antibody phage display libraries, those hits that covalently bind to the proximity site of pBpa on specific epitopes of target antigens after ultraviolet irradiation are enriched and selected. This method was applied to specific epitopes on human interleukin-1β and complement 5a. In both cases, more than one-third of hits identified bind to the target epitopes, demonstrating the feasibility and versatility of this method.
Topics & Concepts
EpitopeAntibodyEpitope mappingSelection (genetic algorithm)Computational biologyAntigenBiologyComputer scienceImmunologyArtificial intelligenceMonoclonal and Polyclonal Antibodies ResearchGlycosylation and Glycoproteins ResearchAdvanced Biosensing Techniques and Applications