Tuning of endosomal escape and gene expression by functional groups, molecular weight and transfection medium: a structure–activity relationship study
Friederike Richter, Liam Martin, Katharina Leer, Elisabeth Moek, Franziska Hausig, Johannes C. Brendel, Anja Traeger
Abstract
is not further protonated inside endosomes, common escape theories appear unsuitable. Therefore, the interaction with bis(monoacryloylglycerol)phosphate, a lipid specific for endosomal vesicles, was investigated. Our research suggests that the interactions between amines and lipids may be more relevant than anticipated.
Topics & Concepts
EndosomeCationic polymerizationTransfectionMoietyPolymerAcrylamidePolymerizationChemistryBiophysicsNucleic acidMonomerCytotoxicityProtonationVesicleRaftBiochemistryCellOrganic chemistryIn vitroBiologyGeneMembraneIonRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesLipid Membrane Structure and Behavior