Enhancement of the Isomerization Activity and Thermostability of Cellobiose 2-Epimerase from <i>Caldicellulosiruptor saccharolyticus</i> by Exchange of a Flexible Loop
Lu Wang, Jiali Gu, Yinghui Feng, Mingming Wang, Yanjun Tong, Yingjie Liu, Xiaomei Lyu, Ruijin Yang
Abstract
C. Our work demonstrated that the isomerization activity and thermostability of CEs were closely related to the flexible loop surrounding the active site, which provides a new perspective to engineer CEs for higher lactulose production.
Topics & Concepts
ThermostabilityChemistryCellobioseIsomerizationMutantActive siteStereochemistryBiochemistryCatalysisEnzymeCellulaseGeneEnzyme Structure and FunctionProtein Structure and DynamicsPancreatic function and diabetes