Ultra-sensitive detection of l-tyrosine using molecularly imprinted electrochemical sensor towards diabetic foot ulcer detection
Souradeep Roy, Shalini Nagabooshanam, Shikha Wadhwa, Nidhi Chauhan, Ashish Mathur, Saif Khan, James Davis
Abstract
It has been found that the concentration of l-tyrosine is greatly elevated within infected ulcers and thus there is a pressing need for sensors that can aid the point of care monitoring of this potential biomarker. In this work, a templated over-oxidised polypyrrole film on indium tin oxide is exploited as a sensitive sensing system, for the detection of tyrosine. The sensor has been characterised using a variety of surface techniques (contact angle, FTIR, SEM) and the electrochemical properties explored using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensor was found to exhibit a substantial linear range (100 fM–1 mM) with limits of detection of 1.73 pM (CV) and 6.63 pM (EIS). The sensor demonstrated rapid response characteristics (less than 1 min) and high stability (up to 3 months).