Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
Martin Peter Rydal, Michela Gambino, Claus B. Jørgensen, Louise Ladefoged Poulsen, Lone Brøndsted, Jens Peter Nielsen
Abstract
Abstract Host genotype is important for enterotoxigenic E. coli (ETEC) susceptibility. We conducted two trials to evaluate the effect of CHCF1 genotype on incidence of ETEC diarrhea. In trial 1 ( n = 15 pigs), pigs were inoculated with 10 8 CFU or 10 10 CFU doses of an ETEC F4ac strain. In trial 2 ( n = 33 pigs), pigs were inoculated with ETEC F4ab or F4ac. Across trials, all inoculated pigs that developed ETEC diarrhea were CHCF1 heterozygous susceptible (6/6). No inoculated CHCF1 homozygous resistant pigs developed ETEC diarrhea (0/26). Susceptibility towards ETEC F4ac/ab infection might correspond with CHCF1 genotype.
Topics & Concepts
Enterotoxigenic Escherichia coliDiarrheaGenotypeEscherichia coliMicrobiologyWeaningBiologyMedicineEnterotoxinAnimal scienceInternal medicineGeneGeneticsEscherichia coli research studiesViral gastroenteritis research and epidemiologyProbiotics and Fermented Foods