CRISPR-Cas12a <i>trans</i>-cleaves DNA G-quadruplexes
Ying Li, Tao Li, Bi‐Feng Liu, Rui Hu, Jiang Zhu, Ting He, Xin Zhou, Conggang Li, Yunhuang Yang, Maili Liu
Abstract
We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.
Topics & Concepts
CRISPRChemistryDNAComputational biologyBiologyGeneBiochemistryCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid Chemistry