Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
Gotaro Toda, Toshimasa Yamauchi, Takashi Kadowaki, Kohjiro Ueki
Abstract
The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).
Topics & Concepts
Bone marrowMacrophageFunction (biology)Protocol (science)Cell biologyBiologyImmunologyPathologyMedicineIn vitroGeneticsAlternative medicineImmune cells in cancerAdipokines, Inflammation, and Metabolic DiseasesExtracellular vesicles in disease