<scp>RecA</scp> levels modulate biofilm development in <i>Acinetobacter baumannii</i>
Carly Ching, Merlin Brychcy, Brian Nguyen, Paul Muller, Alicyn Reverdy Pearson, Margaret Downs, Samuel Regan, Breanna C. Isley, William H. Fowle, Yunrong Chai, Veronica G. Godoy
Abstract
Abstract Infections caused by Acinetobacter baumannii , a Gram‐negative opportunistic pathogen, are difficult to eradicate due to the bacterium's propensity to quickly gain antibiotic resistances and form biofilms, a protective bacterial multicellular community. The A. baumannii DNA damage response (DDR) mediates the antibiotic resistance acquisition and regulates RecA in an atypical fashion; both RecA Low and RecA High cell types are formed in response to DNA damage. The findings of this study demonstrate that the levels of RecA can influence formation and dispersal of biofilms. RecA loss results in surface attachment and prominent biofilms, while elevated RecA leads to diminished attachment and dispersal. These findings suggest that the challenge to treat A. baumannii infections may be explained by the induction of the DDR, common during infection, as well as the delicate balance between maintaining biofilms in low RecA cells and promoting mutagenesis and dispersal in high RecA cells. This study underscores the importance of understanding the fundamental biology of bacteria to develop more effective treatments for infections.